Answered

During the isolation and separation of DNA, what role was played by each of these substances?A) DetergentB) Meat tenderizerC) EthanolD) BufferE) Agarose gelF) Comb

Answer :

Detergent, meat tenderizer, ethanol, buffer and agarose are REAGENTS used to isolate DNA. Moreover, a comb is an INSTRUMENT used to create wells in an agarose/polyacrylamide gel.

Detergent is a reagent that contains sodium laurel sulfate, which is used to remove lipids (fats) and proteins, thereby enabling the isolation of nucleic acids (either DNA or RNA).

The proteinase (or meat tenderizer) is an enzyme that acts to remove proteins, including those that interact with DNA (histones) and nucleases that cleave DNA strands.

Ethanol is a reagent used to achieve the precipitation of the DNA sample in a pellet.

Buffer (e.g., TE buffer) is a reagent used to solubilize DNA while protecting it from its degradation.

Agarose gel is a reagent used to separate DNA fragments according to their size by a technique called electrophoresis.

The comb is an instrument used in an agarose/polyacrylamide gel electrophoresis to create wells where DNA samples are deposited and thus separate them.

Learn more in:

https://brainly.com/question/9437877?referrer=searchResults

Other Questions