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Match each event with the appropriate stage of transcription.The transcription bubble created by RNA polymerase moves along the DNA template.Once bound to the promoter, RNA polymerase begins to unwind the DNA The RNA-DNA hybrid within the transcription bubble dissociates. Reaching a terminator sequence causes formation of phosphodiester bonds to stop. The RNA polymerase holoenzyme is formed.RNA polymerase releases the DNA template. The RNA polymerase holoenzyme binds to the promoter. New nucleotides are added to the 3' end of the growing RNA transcript. RNA polymerase leaves the promoter. A process called clearance or escape.

Answer :

Transcription is a process by which RNA is synthesized from DNA. It is carried out by the enzyme RNA polymerase.

The process is carried out in three steps – Initiation, Elongation and termination.

What steps are followed in Transcription?

A holoenzyme is formed when one or more sigma factor proteins join to the RNA polymerase, allowing it to bind to promoter DNA.

The links between the RNA polymerase holoenzyme and the promoter are broken when the first bond is created in the new RNA molecule.

The transcription bubble formed by RNA polymerase separates the two strands of the DNA helix. This is accomplished by disrupting the hydrogen bonds that exist between complementary DNA nucleotide.

RNA polymerase joins complementary nucleotide of one DNA strand at the 3' OH with corresponding RNA nucleotide.

The RNA sugar-phosphate backbone produces an RNA strand with the help of RNA polymerase.

The untwisted RNA-DNA helix's hydrogen bonds dissolve, releasing the freshly produced RNA strand.

  • The transcription bubble created by RNA polymerase moves along the DNA template. – Elongation
  • Once bound to the promoter, begins to unwind the DNA – Initiation.
  • The RNA-DNA hybrid within the transcription bubble dissociates – Termination.
  • Reaching a terminator sequence causes formation of phosphodiester bonds to stop. – Termination
  • The RNA polymerase holoenzyme is formed. – Initiation
  • RNA polymerase releases the DNA template – Termination.
  • RNA polymerase holoenzyme binds to the promoter – Initiation.
  • New nucleotide are added to the 3’ end of the growing RNA transcript – Elongation.
  • RNA polymerase leaves the promoter. – Elongation.
  • A process called clearance or escape – Elongation.

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